Malaria and the Plasmodium parasite

 

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Infected red blood cells
Malaria is an infection of the blood caused by the tiny protozoan Plasmodium falciparum or Plasmodium vivax.  The Plasmodium parasite is carried by certain species of mosquitoes found in tropical regions across the world.  The bite of the mosquito delivers the parasite into the human blood stream, where the parasite grows first in the liver and then inside the red blood cells.  Soon the infected person has a fever that comes in waves with joint pain, nausea, and eventually may suffer organ failure and secondary brain infection that can be fatal, particularly for small children.  Three hundred million people are infected and more than a million die each year from malaria, most of them children in poor countries.

 

The parasite is becoming resistant to treatment

 

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Location of drug resistant Plasmodium (in yellow).
Malaria can be prevented or treated in a number of ways.  One of the best methods is to avoid being bitten by mosquitoes that carry Plasmodium by using mosquito nets in combination with other mosquito control methods.  For someone already infected, newer drugs are available, but in the countries where malaria is prevalent, the medical system is challenged to deliver these new treatments.  The old treatments with single drugs such as chloroquine have limited effectiveness because drug resistant strains of Plasmodium have now spread around the world.

 

Genomic technology and malaria

 

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A part of the malaria genome
displayed on the Ares lab browser
Genomic technologies developed by studying simple organisms like BakerŐs yeast, the fruit fly, and the nematode worm, as well as more complex organisms like mouse and humans are being applied more and more to parasites that cause disease.  There are good reasons for this.  By learning all about the genes of the Plasmodium parasite we will learn new ways to foil its growth.  To help learn about the genes of Plasmodium, many of which are unknown or only poorly understood, students in the Ares lab have adapted the UCSC genome browser technology to display information about Plasmodium genomes.  We are using the data in our "baby browser" to identify new genes and to make better gene predictions. 

 

 

Looking for new drugs against malaria

 

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UCSC undergraduate Natalie S. grows her cultures of the parasite
We are also about to begin screening small molecules (chemicals) for their ability to inhibit growth of Plasmodium, in an effort to find new potential drugs.  We collaborate with UCSC Professor Scott Lokey on this project, which involves growing many tiny cultures of Plasmodium, each in the presence of a tiny droplet of a different chemical.  We have a collection of more than 10,000 different chemicals to test, some of which may kill the parasite.  If we find such chemicals, more testing will be needed to determine whether they are toxic to human cells in culture, and then animals like mice or rats and then ultimately, if successful, if they are tolerated by humans.  It is a long road and many chemical candidates will fall by the wayside.  But we must look for new treatment methods if we hope to slow or stop this parasite.

 

 

This project has been funded by a grant from the Howard Hughes Medical Institute to develop new ways to combine research and teaching in the undergraduate curriculum.  Funding for this project is scheduled to end in summer of 2006.

 

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